PRECISION USE OF CANNABIS FOR CANCER THERAPY: FROM EPIGENETICS TO EVIDENCE-BASED MEDICINE
“We conducted rigorous testing of Cannabis strains using human cell cultures and 3D tissue models of healthy and diseased tissues. We analyzed anti-cancer, anti-inflammatory and anti-aging properties of Cannabis strains using global transcriptome profiling and an array of cell biology techniques.
Specifically, we tested the extracts for anticancer activity using various breast and colon cancer, glioblastoma, neuroblastoma and other tumor cell lines. We identified a range of active concentrations for these extracts, performed cell cycle arrest analysis and MTT assay, and identified many extracts with significant effect, and established their mechanisms of action via global transcriptome profiling. We have found that in most cases, whole flower extracts were dramatically more efficient than isolated cannabinoids.
We have then analyzed cannabinoid and terpenoids profile of these extracts and attempted to identify common patterns in the presence of specific compounds and the activity of the extracts and identified several correlative patterns. Our data thus indicate that cannabis flower extracts can be very effective in killing cancer cells and that there might be a specific metabolic pattern that is an indicator of potential effectiveness of a given cannabis extract.
Furthermore, using transcriptomic and epigenomic- approach, we have developed CannSelect – an algorithm allowing to select best cannabis extracts for precision medicine applications to treat cancer, aging and a variety of age-related diseases.
Harnessing the many benefits Cannabis can offer through intensive research and development, we hope to improve people’s quality of life, impact longevity and lessen the burden of disease.”